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Nanjing Jiancheng Bioengineering Research Institute Co Ltd giemsa staining kit
In vivo therapeutic effect of PECm-Allicin@LM. A Schematic of the treatment plan. B Peripheral blood smear subjected to <t>Giemsa</t> <t>staining</t> ( Pb ANKA-infected erythrocytes marked with a black arrow). C Survival curves for Pb ANKA-infected ICR mice subjected to different treatments ( n = 10). D Infection rate curves for Pb ANKA-infected ICR mice subjected to different treatments ( n = 10). E Weight curves for ICR mice in different groups ( n = 10). F Red blood cell count in peripheral blood samples from ICR mice in different groups ( n = 6). G Hemoglobin concentration in peripheral blood samples from ICR mice in different groups ( n = 6). H Appearance of livers from ICR mice in different groups. Data are presented as mean ± SEM, and * P < 0.05, ** P < 0.01. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
Giemsa Staining Kit, supplied by Nanjing Jiancheng Bioengineering Research Institute Co Ltd, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/giemsa+staining+kit/assay+blue+brilliant+coomassie+kit/pmc13049907-57-1-7
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1) Product Images from "Allicin-based biomimetic nanoparticles of the erythrocyte membrane for the delivery of lumefantrine to enhance its antimalarial effect"

Article Title: Allicin-based biomimetic nanoparticles of the erythrocyte membrane for the delivery of lumefantrine to enhance its antimalarial effect

Journal: International Journal of Pharmaceutics: X

doi: 10.1016/j.ijpx.2026.100487

In vivo therapeutic effect of PECm-Allicin@LM. A Schematic of the treatment plan. B Peripheral blood smear subjected to Giemsa staining ( Pb ANKA-infected erythrocytes marked with a black arrow). C Survival curves for Pb ANKA-infected ICR mice subjected to different treatments ( n = 10). D Infection rate curves for Pb ANKA-infected ICR mice subjected to different treatments ( n = 10). E Weight curves for ICR mice in different groups ( n = 10). F Red blood cell count in peripheral blood samples from ICR mice in different groups ( n = 6). G Hemoglobin concentration in peripheral blood samples from ICR mice in different groups ( n = 6). H Appearance of livers from ICR mice in different groups. Data are presented as mean ± SEM, and * P < 0.05, ** P < 0.01. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
Figure Legend Snippet: In vivo therapeutic effect of PECm-Allicin@LM. A Schematic of the treatment plan. B Peripheral blood smear subjected to Giemsa staining ( Pb ANKA-infected erythrocytes marked with a black arrow). C Survival curves for Pb ANKA-infected ICR mice subjected to different treatments ( n = 10). D Infection rate curves for Pb ANKA-infected ICR mice subjected to different treatments ( n = 10). E Weight curves for ICR mice in different groups ( n = 10). F Red blood cell count in peripheral blood samples from ICR mice in different groups ( n = 6). G Hemoglobin concentration in peripheral blood samples from ICR mice in different groups ( n = 6). H Appearance of livers from ICR mice in different groups. Data are presented as mean ± SEM, and * P < 0.05, ** P < 0.01. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

Techniques Used: In Vivo, Staining, Infection, Cell Characterization, Concentration Assay

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Staining:

Article Title: CIP2A promotes bronchiolitis obliterans by activating the NF‑κB pathway.
Article Snippet: Bronchiolitis obliterans (BO) is a destructive fibrotic lung disease, which can be partly induced by 2,3‐butanedione [also known as diacetyl (da)]; however, the mechanism under‐ lying the effects of da on Bo is not clear. in the present study, a bioinformatics analysis was performed using da‐treated or untreated lung tissues of rats, and it was observed that cell proliferation regulating inhibitor of protein phosphatase 2a (ciP2a) was significantly increased in samples from the da group. ciP2a is associated with inflammation and epithelial‐mesenchymal transition (eMT), and facilitates lung injury; however, its effect on da‐induced Bo and the under‐ lying mechanism remain unknown.. To solve these issues, da‐treated models of Bo were established in rats and cells, and ethoxysanguinarine (a ciP2a inhibitor) was administered to induce a decrease in ciP2a.. The pathological changes were detected by hematoxylin and eosin, Masson and Giemsa staining. reverse transcription‐quantitative Pcr, western blotting, immunohistochemistry, immunofluorescence and enzyme‐linked immunosorbent assay were used to measure ciP2a expression and levels of pathology‐related markers. notably, inhibition of ciP2a ameliorated the pathological features of Bo, including reduced intraluminal occlusion, inflammatory infiltration and fibrosis.

Article Title: Effect of Oral Peritumoral Tissue on Infiltration and Differentiation of Tumor-Associated Macrophages in Oral Squamous Cell Carcinoma.
Article Snippet: .. After 1 day of incubation at 37 ◦C, 5% carbon dioxide, and 95% air in a humidified environment, the upper chambers (the membranes closer to the lower chamber) were stained with the Giemsa staining kit (Diff-Quick, Nanjing Jiancheng Bioengineering Institute, Nanjing, China), according to the kit protocol. ..

Article Title: Crosstalk between cancer and different cancer stroma subtypes promotes the infiltration of tumor-associated macrophages into the tumor microenvironment of oral squamous cell carcinoma
Article Snippet: .. The RAW264.7 cells were seeded into the upper chamber of 8- μ m Transwell filters without Matrigel in 24-well plates (Corning, Falcon cell culture inserts; BD Biosciences) in α-MEM without FBS at a density of 2×10 4 cells/500 μ l. Following incubation for 1 day at 37°C in a humidified atmosphere of 5% CO 2 and 95% air, the upper chambers were stained using the Giemsa staining kit (Diff-Quick, Nanjing Jiancheng Bioengineering Institute). ..

Article Title: Effect of Oral Peritumoral Tissue on Infiltration and Differentiation of Tumor-Associated Macrophages in Oral Squamous Cell Carcinoma
Article Snippet: .. After 1 day of incubation at 37 °C, 5% carbon dioxide, and 95% air in a humidified environment, the upper chambers (the membranes closer to the lower chamber) were stained with the Giemsa staining kit (Diff-Quick, Nanjing Jiancheng Bioengineering Institute, Nanjing, China), according to the kit protocol. ..

Article Title: Stromal cells in the tumor microenvironment promote the progression of oral squamous cell carcinoma
Article Snippet: The cells were seeded into small dishes (Violamo, 35×12 mm) with a slide (Matsunami, 22×22 mm) at a density of 4×10 5 /dish. .. Following incubation for 1 day (37°C), the attached slides were stained using the Giemsa Staining kit (Diff-Quick; Nanjing Jiancheng Bioengineering Institute). ..

Article Title: CIP2A promotes bronchiolitis obliterans by activating the NF‑κB pathway
Article Snippet: .. Giemsa staining at room temperature for 1 min with Giemsa A and for 7 min with Giemsa B (Giemsa Staining Kit; Nanjing Jiancheng Bioengineering Institute) was carried out to manually measure the number of different types of cells under a DP73 fluorescence microscope (Olympus Corporation), including macrophages, lymphocytes, neutrophils and eosinophils. ..

Article Title: Allicin-based biomimetic nanoparticles of the erythrocyte membrane for the delivery of lumefantrine to enhance its antimalarial effect
Article Snippet: .. A Giemsa staining kit was purchased from Nanjing Jiancheng Bioengineering Research Institute Co., Ltd. (China). .. Tween-80 was purchased from Shanghai Titan Scientific Co., Ltd. (China).

Fluorescence:

Article Title: CIP2A promotes bronchiolitis obliterans by activating the NF‑κB pathway.
Article Snippet: Bronchiolitis obliterans (BO) is a destructive fibrotic lung disease, which can be partly induced by 2,3‐butanedione [also known as diacetyl (da)]; however, the mechanism under‐ lying the effects of da on Bo is not clear. in the present study, a bioinformatics analysis was performed using da‐treated or untreated lung tissues of rats, and it was observed that cell proliferation regulating inhibitor of protein phosphatase 2a (ciP2a) was significantly increased in samples from the da group. ciP2a is associated with inflammation and epithelial‐mesenchymal transition (eMT), and facilitates lung injury; however, its effect on da‐induced Bo and the under‐ lying mechanism remain unknown.. To solve these issues, da‐treated models of Bo were established in rats and cells, and ethoxysanguinarine (a ciP2a inhibitor) was administered to induce a decrease in ciP2a.. The pathological changes were detected by hematoxylin and eosin, Masson and Giemsa staining. reverse transcription‐quantitative Pcr, western blotting, immunohistochemistry, immunofluorescence and enzyme‐linked immunosorbent assay were used to measure ciP2a expression and levels of pathology‐related markers. notably, inhibition of ciP2a ameliorated the pathological features of Bo, including reduced intraluminal occlusion, inflammatory infiltration and fibrosis.

Article Title: CIP2A promotes bronchiolitis obliterans by activating the NF‑κB pathway
Article Snippet: .. Giemsa staining at room temperature for 1 min with Giemsa A and for 7 min with Giemsa B (Giemsa Staining Kit; Nanjing Jiancheng Bioengineering Institute) was carried out to manually measure the number of different types of cells under a DP73 fluorescence microscope (Olympus Corporation), including macrophages, lymphocytes, neutrophils and eosinophils. ..

Microscopy:

Article Title: CIP2A promotes bronchiolitis obliterans by activating the NF‑κB pathway.
Article Snippet: Bronchiolitis obliterans (BO) is a destructive fibrotic lung disease, which can be partly induced by 2,3‐butanedione [also known as diacetyl (da)]; however, the mechanism under‐ lying the effects of da on Bo is not clear. in the present study, a bioinformatics analysis was performed using da‐treated or untreated lung tissues of rats, and it was observed that cell proliferation regulating inhibitor of protein phosphatase 2a (ciP2a) was significantly increased in samples from the da group. ciP2a is associated with inflammation and epithelial‐mesenchymal transition (eMT), and facilitates lung injury; however, its effect on da‐induced Bo and the under‐ lying mechanism remain unknown.. To solve these issues, da‐treated models of Bo were established in rats and cells, and ethoxysanguinarine (a ciP2a inhibitor) was administered to induce a decrease in ciP2a.. The pathological changes were detected by hematoxylin and eosin, Masson and Giemsa staining. reverse transcription‐quantitative Pcr, western blotting, immunohistochemistry, immunofluorescence and enzyme‐linked immunosorbent assay were used to measure ciP2a expression and levels of pathology‐related markers. notably, inhibition of ciP2a ameliorated the pathological features of Bo, including reduced intraluminal occlusion, inflammatory infiltration and fibrosis.

Article Title: CIP2A promotes bronchiolitis obliterans by activating the NF‑κB pathway
Article Snippet: .. Giemsa staining at room temperature for 1 min with Giemsa A and for 7 min with Giemsa B (Giemsa Staining Kit; Nanjing Jiancheng Bioengineering Institute) was carried out to manually measure the number of different types of cells under a DP73 fluorescence microscope (Olympus Corporation), including macrophages, lymphocytes, neutrophils and eosinophils. ..

Incubation:

Article Title: Effect of Oral Peritumoral Tissue on Infiltration and Differentiation of Tumor-Associated Macrophages in Oral Squamous Cell Carcinoma.
Article Snippet: .. After 1 day of incubation at 37 ◦C, 5% carbon dioxide, and 95% air in a humidified environment, the upper chambers (the membranes closer to the lower chamber) were stained with the Giemsa staining kit (Diff-Quick, Nanjing Jiancheng Bioengineering Institute, Nanjing, China), according to the kit protocol. ..

Article Title: Crosstalk between cancer and different cancer stroma subtypes promotes the infiltration of tumor-associated macrophages into the tumor microenvironment of oral squamous cell carcinoma
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Article Title: Effect of Oral Peritumoral Tissue on Infiltration and Differentiation of Tumor-Associated Macrophages in Oral Squamous Cell Carcinoma
Article Snippet: .. After 1 day of incubation at 37 °C, 5% carbon dioxide, and 95% air in a humidified environment, the upper chambers (the membranes closer to the lower chamber) were stained with the Giemsa staining kit (Diff-Quick, Nanjing Jiancheng Bioengineering Institute, Nanjing, China), according to the kit protocol. ..

Article Title: Stromal cells in the tumor microenvironment promote the progression of oral squamous cell carcinoma
Article Snippet: The cells were seeded into small dishes (Violamo, 35×12 mm) with a slide (Matsunami, 22×22 mm) at a density of 4×10 5 /dish. .. Following incubation for 1 day (37°C), the attached slides were stained using the Giemsa Staining kit (Diff-Quick; Nanjing Jiancheng Bioengineering Institute). ..

Diff-Quik:

Article Title: Effect of Oral Peritumoral Tissue on Infiltration and Differentiation of Tumor-Associated Macrophages in Oral Squamous Cell Carcinoma.
Article Snippet: .. After 1 day of incubation at 37 ◦C, 5% carbon dioxide, and 95% air in a humidified environment, the upper chambers (the membranes closer to the lower chamber) were stained with the Giemsa staining kit (Diff-Quick, Nanjing Jiancheng Bioengineering Institute, Nanjing, China), according to the kit protocol. ..

Article Title: Crosstalk between cancer and different cancer stroma subtypes promotes the infiltration of tumor-associated macrophages into the tumor microenvironment of oral squamous cell carcinoma
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Article Title: Effect of Oral Peritumoral Tissue on Infiltration and Differentiation of Tumor-Associated Macrophages in Oral Squamous Cell Carcinoma
Article Snippet: .. After 1 day of incubation at 37 °C, 5% carbon dioxide, and 95% air in a humidified environment, the upper chambers (the membranes closer to the lower chamber) were stained with the Giemsa staining kit (Diff-Quick, Nanjing Jiancheng Bioengineering Institute, Nanjing, China), according to the kit protocol. ..

Article Title: Stromal cells in the tumor microenvironment promote the progression of oral squamous cell carcinoma
Article Snippet: The cells were seeded into small dishes (Violamo, 35×12 mm) with a slide (Matsunami, 22×22 mm) at a density of 4×10 5 /dish. .. Following incubation for 1 day (37°C), the attached slides were stained using the Giemsa Staining kit (Diff-Quick; Nanjing Jiancheng Bioengineering Institute). ..

Cell Culture:

Article Title: Crosstalk between cancer and different cancer stroma subtypes promotes the infiltration of tumor-associated macrophages into the tumor microenvironment of oral squamous cell carcinoma
Article Snippet: .. The RAW264.7 cells were seeded into the upper chamber of 8- μ m Transwell filters without Matrigel in 24-well plates (Corning, Falcon cell culture inserts; BD Biosciences) in α-MEM without FBS at a density of 2×10 4 cells/500 μ l. Following incubation for 1 day at 37°C in a humidified atmosphere of 5% CO 2 and 95% air, the upper chambers were stained using the Giemsa staining kit (Diff-Quick, Nanjing Jiancheng Bioengineering Institute). ..



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In vivo therapeutic effect of PECm-Allicin@LM. A Schematic of the treatment plan. B Peripheral blood smear subjected to <t>Giemsa</t> <t>staining</t> ( Pb ANKA-infected erythrocytes marked with a black arrow). C Survival curves for Pb ANKA-infected ICR mice subjected to different treatments ( n = 10). D Infection rate curves for Pb ANKA-infected ICR mice subjected to different treatments ( n = 10). E Weight curves for ICR mice in different groups ( n = 10). F Red blood cell count in peripheral blood samples from ICR mice in different groups ( n = 6). G Hemoglobin concentration in peripheral blood samples from ICR mice in different groups ( n = 6). H Appearance of livers from ICR mice in different groups. Data are presented as mean ± SEM, and * P < 0.05, ** P < 0.01. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
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Higher EOF in patients with glaucoma . ( a ) Flowchart of the EOFT. ( b ) Statistical results show the average concentration of NaCl at which RBCs displayed complete hemolysis (maximum resistance). The data are expressed as mean ± SEM. **** P < 0.0001; unpaired t -test. ( c ) Histogram of the NaCl concentrations of maximum resistance for patients with glaucoma and healthy controls. Each dot represents one patient with glaucoma or healthy control. ( d ) Representative image showing <t>Giemsa</t> <t>staining</t> of RBCs from patients with glaucoma and healthy controls. The data are expressed as mean ± SEM. **** P < 0.0001; unpaired t -test. ( e ) Quantification of the Giemsa staining result showing the percentage of abnormal RBCs in patients with glaucoma and healthy controls. ( f ) EOFT results for patients A and B with glaucoma and healthy control. ( g–h ) OCT results of patients A and B. INF, inferior; NAS, nasal; OD, right eye; OS, left eye; RNFL, retinal nerve fiber layer; SUP, superior; TEMP, temporal.
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Image Search Results


In vivo therapeutic effect of PECm-Allicin@LM. A Schematic of the treatment plan. B Peripheral blood smear subjected to Giemsa staining ( Pb ANKA-infected erythrocytes marked with a black arrow). C Survival curves for Pb ANKA-infected ICR mice subjected to different treatments ( n = 10). D Infection rate curves for Pb ANKA-infected ICR mice subjected to different treatments ( n = 10). E Weight curves for ICR mice in different groups ( n = 10). F Red blood cell count in peripheral blood samples from ICR mice in different groups ( n = 6). G Hemoglobin concentration in peripheral blood samples from ICR mice in different groups ( n = 6). H Appearance of livers from ICR mice in different groups. Data are presented as mean ± SEM, and * P < 0.05, ** P < 0.01. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

Journal: International Journal of Pharmaceutics: X

Article Title: Allicin-based biomimetic nanoparticles of the erythrocyte membrane for the delivery of lumefantrine to enhance its antimalarial effect

doi: 10.1016/j.ijpx.2026.100487

Figure Lengend Snippet: In vivo therapeutic effect of PECm-Allicin@LM. A Schematic of the treatment plan. B Peripheral blood smear subjected to Giemsa staining ( Pb ANKA-infected erythrocytes marked with a black arrow). C Survival curves for Pb ANKA-infected ICR mice subjected to different treatments ( n = 10). D Infection rate curves for Pb ANKA-infected ICR mice subjected to different treatments ( n = 10). E Weight curves for ICR mice in different groups ( n = 10). F Red blood cell count in peripheral blood samples from ICR mice in different groups ( n = 6). G Hemoglobin concentration in peripheral blood samples from ICR mice in different groups ( n = 6). H Appearance of livers from ICR mice in different groups. Data are presented as mean ± SEM, and * P < 0.05, ** P < 0.01. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

Article Snippet: A Giemsa staining kit was purchased from Nanjing Jiancheng Bioengineering Research Institute Co., Ltd. (China).

Techniques: In Vivo, Staining, Infection, Cell Characterization, Concentration Assay

Higher EOF in patients with glaucoma . ( a ) Flowchart of the EOFT. ( b ) Statistical results show the average concentration of NaCl at which RBCs displayed complete hemolysis (maximum resistance). The data are expressed as mean ± SEM. **** P < 0.0001; unpaired t -test. ( c ) Histogram of the NaCl concentrations of maximum resistance for patients with glaucoma and healthy controls. Each dot represents one patient with glaucoma or healthy control. ( d ) Representative image showing Giemsa staining of RBCs from patients with glaucoma and healthy controls. The data are expressed as mean ± SEM. **** P < 0.0001; unpaired t -test. ( e ) Quantification of the Giemsa staining result showing the percentage of abnormal RBCs in patients with glaucoma and healthy controls. ( f ) EOFT results for patients A and B with glaucoma and healthy control. ( g–h ) OCT results of patients A and B. INF, inferior; NAS, nasal; OD, right eye; OS, left eye; RNFL, retinal nerve fiber layer; SUP, superior; TEMP, temporal.

Journal: Translational Vision Science & Technology

Article Title: Increased Erythrocyte Osmotic Fragility as a Risk Factor for Predicting Glaucoma

doi: 10.1167/tvst.15.2.4

Figure Lengend Snippet: Higher EOF in patients with glaucoma . ( a ) Flowchart of the EOFT. ( b ) Statistical results show the average concentration of NaCl at which RBCs displayed complete hemolysis (maximum resistance). The data are expressed as mean ± SEM. **** P < 0.0001; unpaired t -test. ( c ) Histogram of the NaCl concentrations of maximum resistance for patients with glaucoma and healthy controls. Each dot represents one patient with glaucoma or healthy control. ( d ) Representative image showing Giemsa staining of RBCs from patients with glaucoma and healthy controls. The data are expressed as mean ± SEM. **** P < 0.0001; unpaired t -test. ( e ) Quantification of the Giemsa staining result showing the percentage of abnormal RBCs in patients with glaucoma and healthy controls. ( f ) EOFT results for patients A and B with glaucoma and healthy control. ( g–h ) OCT results of patients A and B. INF, inferior; NAS, nasal; OD, right eye; OS, left eye; RNFL, retinal nerve fiber layer; SUP, superior; TEMP, temporal.

Article Snippet: The A Giemsa staining kit (Yeasen, China) was used to stain the smear following the manufacturer's instructions.

Techniques: Concentration Assay, Control, Staining